Notes for 11/13/01

Muscle

 

Threshold Stimulus

All-or-None response- in order for the sarcolemma to depolarize, and thus result in a contraction a certain stimulus strength (threshold stimulus) must be achieved.  If the stimulus strength falls short of this threshold, the chain-reaction will not occur and Ca++ will not be released from the cisternae of the sarcoplasmic reticulum.   If the stimulus strength is much greater than the threshold, the muscle fiber response (strength and speed of contraction) will be the same as if the threshold was just barely met.

 

Think of it like a light switch.  It doesn’t matter how strong or fast you turn on a light switch, the light from the bulb is the same.  If you want to have more light in the room you have to turn on more bulbs.

 

·        The same with the strength of muscle contraction.  If you want more strength you must activate a greater number of muscle fibers.  That means more motor units must be recruited, and thus more muscle fibers will be involved in the contraction.

 

Latent Period- time lag between application of stimulus (membrane depolarization) and when the contraction occurs.  This time lag can be attributed to the amount of time it takes for Ca++ to diffuse out of the sarcoplasmic reticulum, bind to troponin, for troponin to remove tropomyosin from the active sites on Actin, and for cross-bridges to form.

 

Period of Contraction- time period during which muscle fiber contracts

 

Period of Relaxation- time after muscle fiber contracts.

 

Refractory Period- the membrane depolarization that causes Ca++ to diffuse out of the sarcoplasmic reticulum is set up by a concentration gradiant of Na+ on the outside of the cell and K+ on the inside of the cell.

 

·        When depolarized, membrane proteins open channels that cause Na and K to diffuse down their chemical gradients, until the membrane proteins close the channels again.

·        Then it takes time for the active transport pumps to bring Na back into the cell and K out of the cell.  Until this happens, no further muscle contraction can be induced and this is called the refractory period.

 

Tetany- When Ca++ remain at high concentrations with the sarcoplasm for extended periods, the muscle fiber will not relax, but maintains a sustained contraction.  This can occur normally, when the depolarization stimulus of the sarcolemma occurs at high rates, and is in fact faster than the Ca++ active transport pumps can sequester the Ca++ back into the sarcoplasmic reticulum.  Thus, more and more Ca++ can build up in the sarcoplasm and result in maximal muscle-fiber contraction (called summation).

 

Even while at “rest”, a muscle fiber undergoes low levels of contraction resulting in muscle tone.  Period signal from the nervous system maintain muscle tone and these are especially important in postural muscles.

 

Note:  if the innervation to muscle fibers is cut or lost, muscle tone will be lost and eventually the muscle will atrophy from loss of use.  The body does not maintain structures that are not being used.

 

Types of Muscle Fibers

 Slow-twitch fibers (Type I)- oxidative metabolism, resistant to fatigue, high amounts in postural muscles (back), deep red in appearance due to high concentrations of myoglobin bound with Oxygen.  Lots of mitochondria are present for generation of ATP via Citric Acid cycle and Electron Transport Chain.

 

Fast-twitch glycolytic fibers (Type IIa)- white in appearance because of lesser amounts of myoglobin, and poorer blood supply.  Reduced respiratory capacity.  Found in high concentrations in muscles of rapid movement, hands and oculomotor muscles.  Faster ATPase activity and higher concentrations of store Ca++ (steeper chemical gradient gives faster diffusion rate and faster contraction speed).  These muscles do fatigue

 

Fast-twitch fatique resistant fibers (Type II b)- intermediate properties between Type I and II a.

 

All muscles consist of combinations of these fiber types, with varying concentrations of the different types depending on the function.

 

Smooth Muscle-

Shorter than skeletal muscle and have centrally located nucleus

Not a syncytium like skeletal muscle

Lack Transverse tubules and extensive Sarcoplasmic reticulum.

Fewer myofibrils, and not all arranged in parallel (non-striated)

 

Multiunit smooth muscle- not well organized and each cell is independent of the others (has independent innervation).  Found in iris of eye and in walls of blood vessels

 

Visceral smooth muscle- sheets of spindle-shaped cells interconnected via gap junctions (recall this permits the communication of cytoplasm)

Cells respond as single unit, that is they coordinate contraction.  When one cell is stimulated its neighbors quickly thereafter become stimulated.  Found in tubular organs like Gastrointestinal tract.

 

Peristalsis- wavelike muscle contraction within tubular organs that move contents along.

 

Contraction-

·        Calmodulin used in place of troponin.  Ca++ comes from extracellular fluid rather than within the sarcoplasmic reticulum.

·        Neurotransmitters include acetylcholine and norepinephrine.  These stimulate or inhibit contraction by binding to membrane post-synaptic membrane proteins.

·        Hormones also exhibit a stimulatory or inhibitory effect.  Stretching the muscle can also stimulate contraction.

·        Slower contraction than skeletal muscle, but more sustained contraction for a given amount of ATP

 

Cardiac Muscle- branched, striated cells attached end to end.  (Intercalated disks mark the boundaries between cells).  More similar to skeletal muscle, except the cisternae of sarcoplasmic reticulum are less well developed and store less Ca++.  Ca++ comes from extracellular fluid via deeper penetration of the transverse tubules.

 

Intercalated disks are specialized gap junctions that allow ions to diffuse between cells, and cause membrane depolarization to travel and branch to other cells.  This results in a coordinated contraction and relaxation of cardiac muscle.

There are specialized areas of cardiac muscle called the Sinoatrial node and the Atrioventricular node that are made of cardiac muscle with leaky membranes to Ca++.  This causes a self-exciting and rhythmic stimulus for contraction, and explains why a heart can continue to beat in the absence of innervation.